附註:Includes bibliographical references (pages 595-658) and index.
Getting started -- Types of nerve cell cultures, their advantages and limitations -- Primary dissociated cell cultures -- Transfecting cultured neurons -- Characterizing and studying neuronal cultures -- Culture and experimental use of the PC12 rat pheochromocytoma cell line -- Neuronlike cells derived in culture from P19 embryonal carcinoma and embryonic stem cells -- Culturing the large neurons of aplysia -- Culturing spinal neurons and muscle cells from Xenopus embryos -- Cultures from chick peripheral ganglia -- Culturing mammalian sympathoadrenal derivatives -- Mass cultures and microislands of neurons from postnatal rat brain -- Rat hippocampal neurons in low-density culture -- Rat striatal neurons in low-density, serum-free culture -- Cell culture of cholinergic and cholinoceptive neurons from the medial habenula -- The Cerebellum : purification and coculture of identified cell populations -- Organotypic slice cultures of neural tissue -- Culture of astrocytes, oligodendrocytes, and O-2A progenitor cells -- Tissue culture methods for the study of myelination.
摘要:Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit. The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. Contributors : Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F.A. Finley, Gerald D. Fishbach, Beat H. Gähwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Pröschel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T. Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.
