附註:DNA analysis by gel electrophoresis -- Plasmid isolation -- Prokaryotic chromosomal DNA -- Eukaryotic chromosomal DNA -- Restriction enzyme digestion and size determination of DNA -- Isolation of restriction enzymes -- Restriction-modification of DNA -- Construction and mapping of a recombinant plasmid -- DNA fingerprinting -- Aseptic techniques : staining and culturing bacteria -- Transformation of E. coli -- Preparation of competent E. coli cells for transformation -- Spontaneous mutation : direct selection -- Spontaneous mutation : indirect selection -- Induced mutation -- In-vitro mutagenesis -- DNA sequencing -- PCR amplification -- Preparation of biotinylated probe lambda DNA -- Southern blot analysis -- Gel filtration chromatography -- Ion exchange chromatography -- Affinity chromatography -- Purification of wheat germ phosphatase -- Chromatographic desalting of proteins.
