Spin labeling :the next millennium

  • 其他作者: Berliner, Lawrence J.
  • 出版: New York : Kluwer Academic ©2002.
  • 稽核項: 1 online resource (xvii, 423 pages) :illustrations.
  • 叢書名: Biological magnetic resonance ;v. 14
  • 標題: Résonance magnétique. , Technique. , Biologie moléculaire Technique. , Molecular biology Technique. , Molecular biology , SCIENCE Life Sciences -- Biochemistry. , SCIENCE , Marqueurs de spin. , Life SciencesBiochemistry. , Biologie moléculaire , Electronic books. , Magnetic resonance. , Spin labels. , Spin Labels
  • ISBN: 6610206430 , 9786610206438
  • ISBN: 0306456443 , 9780306456442
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  • 附註: Includes bibliographical references and index. Cover -- Table of Contents -- Introduction: Reflections on the Beginning of the Spin Labeling Technique -- Chapter 1 Analysis of Spin Label Line Shapes with Novel Inhomogeneous Broadening from Different Component Widths: Application to Spatially Disconnected Domains in Membranes -- 1. Introduction -- 1.1. Inhomogeneous Broadening Due to Unresolved Hyperfine Structure -- 1. 2. Inhomogeneous Broadening Due to Different Component Widths . -- 2. Theory -- 2.1. Sum of Two Spectra with Different Widths -- 2.2. Statistical Distribution of Spin Labels -- 2.3. Methods to Determine the Existence of a Statistical Distribution of Spin Labels -- 2.4. Experimental Considerations -- 3. Applications -- 3.1. Domain Structure in a Two-Component, Two-Phase Lipid Bilayer System -- 3.2. Effect of a Peptide on Domain Structure -- 4. Conclusions -- References -- Chapter 2 Progressive Saturation and Saturation Transfer EPR for Measuring Exchange Processes and Proximity Relations in Membranes -- 1. Introduction -- 1.1. Continuous Wave Saturation -- 1. 2. Effective Spin Lattice Relaxation Times -- 2. Contributions to Intrinsic Spin-Lattice Relaxation Rates -- 2.1. Electron Relaxation Mechanisms -- 2.2. Nuclear Relaxation Mechanisms -- 3. Paramagnetic Relaxation Enhancement -- 3.1. Paramagnetic Enhancement by Heisenberg Exchange -- 3.2. Paramagnetic Enhancement by Magnetic Dipole-Dipole Interactions -- 4. Spin Lattice Relaxation Enhancement by Exchange Processes -- 4.1. Two-Site Exchange -- 4.2. Heisenberg Spin Exchange -- 5. Continuous Wave Saturation Measurements -- 5.1. Progressive Saturation EPR -- 5.2. Saturation Transfer EPR -- 6. Applications -- 6.1. Two-Site Lipid Exchange and Lipid-Protein Interactions -- 6.2. Lipid and Protein Collision Frequencies: Translational Diffusion -- 6.3. Spin Label Location and Accessibility: Paramagnetic Relaxation -- 6.4. Distance Measurements: Dipolar Relaxation -- 6.5. Slow Rotational Diffusion and Saturation Studies -- 7. Conclusions -- References
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