附註:Includes bibliographical references and index.
Cover; Inside Cover; Copyright; Table of Contents; Contributors; Foreword; Section I Biochemistry of Ribozymes; CHAPTER 1 The Biochemistry of the Hammerhead Ribozyme; CHAPTER 2 Biochemistry of the Hairpin Ribozyme; CHAPTER 3 Biochemistry of Hepatitis Delta Virus Catalytic RNAs; Section II Expression and Delivery of Ribozymes; CHAPTER 4 Exogenous Delivery of Ribozymes; CHAPTER 5 Novel RNA Motif (Dimeric Minizyme) Capable of Cleaving L6 BCR-ABL Fusion (b2a2) mRNA with High Specificity; CHAPTER 6 Using Ribozymes to Attenuate Gene Expression in Transgenic Mice.
摘要:This book is useful for understanding the essential methods for developing therapeutic applications of ribozymes to disease genes. Detailed protocols are used to design, synthesize and apply ribozymes to knock out disease-associated genes. The primary target for ribozymes is on cancer genes, however, the application can be used for any type of gene.
