附註:Includes bibliographical references and index.
A primer on transcriptional regulation in mammalian cells -- Initial strategic issues -- Modes of regulating mRNA abundance -- Transcription initiation site mapping -- Functional assays for promoter analysis -- Identification and analysis of distant control regions -- Identifying cis-acting DNA elements within a control region -- Identification of DNA-binding proteins and isolation of their genes -- Confirming the functional importance of a protein-DNA interaction -- In vivo analysis of an endogenous control region -- Approaches for the synthesis of recombinant transcription factors -- Identifying and characterizing transcription factor domains -- Theory, characterization, and modeling of DNA binding by regulatory transcription factors -- Crude and fractionated systems for in vitro transcription -- Approaches for studying transcription complex assembly.
摘要:"In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene or the biochemistry of a new transcription factor." "This book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states."--Jacket.
