附註:Includes bibliographical references (pages 243-245) and index.
Cover -- Table of Contents -- Preface -- 1. Laws of Physics and Chemistry -- 1.1 Introduction -- 1.2 Quantum Mechanics -- 1.3 The Electronic Structure of Atoms -- 1.4 Molecular Orbitals and Covalent Bonds -- 1.5 Molecular Interactions -- 1.6 Stereochemistry and Chirality -- 1.7 Thermodynamics -- 1.8 Radioactivity -- 2. Separation Techniques -- 2.1 Introduction -- 2.2 Chromatography -- 2.3 Electrophoresis -- 3. Physico-Chemical Techniques to Study Biomolecules -- 3.1 Introduction -- 3.2 Hydration of Macromolecules -- 3.3 Role of Friction -- 3.4 Diffusion -- 3.5 Sedimentation -- 3.6 The Ultracentrifuge -- 3.7 Viscosity -- 3.8 Rotational Diffusion -- 3.9 Light Scattering -- 3.10 Small Angle X-ray Scattering -- 4. Spectroscopy -- 4.1 Introduction -- 4.2 Ultraviolet/Visible Spectroscopy -- 4.3 Circular Dichroism (CD) and Optical Rotatory Dispersion (ORD) -- 4.4 Fluorescence Spectroscopy -- 4.5 Infrared Spectroscopy -- 4.6 Raman Spectroscopy -- 4.7 Electron Spin Resonance -- 5. Light Microscopy -- 5.1 Introduction -- 5.2 Elementary Geometrical Optics -- 5.3 The Limits of Resolution -- 5.4 Different Types of Microscopy -- 6. Electron Microscopy -- 6.1 Introduction -- 6.2 Electron Optics -- 6.3 The Transmission Electron Microscope (TEM) -- 6.4 The Scanning Electron Microscope (SEM) -- 6.5 Preparation of the Specimen for Electron Microscopy -- 6.6 Image Reconstruction -- 6.7 Electron Diffraction -- 6.8 The Tunnelling Electron Microscope -- 6.9 Atomic Force Microscope -- 7. X-ray Crystallography -- 7.1 Introduction -- 7.2 Crystals and Symmetries -- 7.3 Crystal Systems -- 7.4 Point Groups and Space Groups -- 7.5 Growth of Crystals of Biological Molecules -- 7.6 X-ray Diffraction -- 7.7 X-ray Data Collection -- 7.8 Structure Solution -- 7.9 Refinement of the Structure -- 7.10 Note on the Resolution of an X-ray Structure -- 8. NMR Spectroscopy -- 8.1 Introduction -- 8.2 Basic Principles of NMR -- 8.3 NMR Theory and Experiment -- 8.4 Classical Description of NMR -- 8.5 NMR Par
摘要:Biophysics, being an interdisciplinary topic, is of great importance in modern biology. This book addresses the needs of biologists, biochemists, and medical biophysicists for an introduction to the subject. The text is based on a one-semester course offered to graduate students of life sciences, and covers a wide range of topics from quantum mechanics to pre-biotic evolution. To understand the topics, only basic school level mathematics is required. The first chapter introduces and refreshes the reader's knowledge of physics and chemistry. The next chapters cover various physico-chemical techniques used to study biomolecular structures, followed by treatments of spectroscopy, microscopy, diffraction, and computational techniques. X-ray crystallography and NMR are dealt with in greater detail. The latter half of the book covers results obtained from applications of the above techniques. Some of the other topics dealt with are energy pathways, biomechanics, and neuro-biophysics.
