Microscopy, immunohistochemistry, and antigen retrieval methods :for light and electron microscopy

• 作者: Hayat, M. A.,
• 出版: New York : Kluwer Academic/Plenum Publishers ©2002.
• 稽核項: 1 online resource (xviii, 355 pages) :color illustrations.
• 標題: isolation & purification , Immunohistochemistry , Electron microscopy. , MEDICAL , Electronic books. , Antigens. , Microscopy , Antigens isolation & purification , Histology. , Antigens , MEDICAL Histology. , Microscopy. , Immunohistochemistry.
• ISBN: 0306467704 , 9780306467707
• ISBN: 9786610200870 , 6610200874 , 1280200871 , 9781280200878
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• 附註: Includes bibliographical references (pages 305-350) and index. Cover -- Preface -- Table of Contents -- Chapter 1. Introduction -- Cytogenetic Evaluation of Tumors -- Genetic Instability of Tumors -- Tumor Heterogeneity -- Antitumor Vaccines -- Molecular Genetics -- cDNA Microarray Technology -- Angiogenesis -- Future of Immunohistopathology -- Preparation of Buffers -- Chapter 2. Antigens and Antibodies -- Antigens -- Antibodies -- Recombinant Antibodies -- Anticancer Monoclonal Antibodies -- Antibody Cross-Reactivity -- Polyreactive Antibodies -- Commercial Sources of Antibodies -- Chapter 3. Fixation and Embedding -- Formaldehyde -- Effect of Heating on Fixation with Glutaraldehyde -- Microwave Heat ... Assisted Fixation with Osmium Tetroxide -- Role of Microwave Heating in Enzyme Cytochemistry -- Cryopreservation in the Presence of Microwave Heating -- Paraffin Embedding -- Chapter 4. Factors Affecting Antigen Retrieval -- Fixation -- Denaturation -- Heating -- pH -- Molarity -- Antigen Retrieval Fluids -- Antibody Penetration -- Antibody Dilution -- Storage of Paraffin-Embedded Tissues -- Storage of Tissue Slides -- Signal Amplification -- Chapter 5. Problems in Antigen Retrieval -- Lack of Immunostaining -- Background Staining -- Problem of Endogenous Biotin -- Mirror Image Complementary Antibodies -- Fixation of Frozen Tissues -- Hot Spots (Areas) in Microwave Oven -- Problem of Antigen Retrieval Standardization -- Test Battery -- Intraobserver and Interobserver Variation in Diagnosis -- Quantitation of Immunostaining -- Autostainers -- Volume-Corrected Mitotic Index -- The Gleason Grading System -- Universal Antigen Retrieval Method? -- Calibration of Microwave Oven -- Chapter 6. Antigen Retrieval -- Possible Mechanisms of Antigen Retrieval -- Effect of Endogenous Calcium on Antigen Masking -- Use of Ethylenediaminetetraacetic Acid (EDTA) for Antigen Retrieval -- Antigen Retrieval with Heat Treatment -- Advantages of Heating -- Heating Methods -- Wet Autoclave Method -- Ultrasound Treatment -- Nonheating Methods -- C
• 摘要: Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
• 電子資源: https://dbs.tnua.edu.tw/login?url=https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=78506
• 系統號: 005300521
• 資料類型: 電子書
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• 引用網址: 複製連結
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