Membrane transport :a practical approach
- 其他作者: Baldwin, Stephen A.
- 出版: Oxford ;New York : Oxford University Press 2000.
- 稽核項: 1 online resource (xxi, 320 pages) :illustrations.
- 叢書名: The practical approach series ;230
- 標題: Carrier proteins , Membranas celulares. , Membrantransport , Carrier proteins. , Transporte através da membrana. , Cell membranes , Cytological Techniques , Eiwitten. , Carrier Proteins physiology , Carrier proteins Experiments. , Laboratory Manual , physiology , Protein Transport , Membranen. , Cell membranes Experiments. , SCIENCE , Experiments. , Cell Membrane , Biomoleculen. , Protein Transport physiology , Electronic books. , Transportverschijnselen. , Biological transport Laboratory manuals. , Life SciencesBiochemistry. , Carrier Proteins , SCIENCE Life Sciences -- Biochemistry. , Biological transport , Cell Membrane physiology , Cells , Biological transport. , metabolism , Carrier proteins Laboratory manuals. , Cells metabolism , Laboratory manuals.
- ISBN: 0191565822 , 9780191565823
- 試查全文@TNUA:
- 附註: Includes bibliographical references and index. Assay of membrane transport in cells and membrane vesicles / Simon M. Jarvis -- Reconstitution of membrane proteins : the Ca²-ATPase of sarcoplasmic reticulum / Anthony G. Lee -- The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins / Sylvia Y.M. Yao, Carol E. Cass, and James D. Young -- Expression of foreign transport proteins in yeast / N. Sauer and J. Stolz -- Baculovirus-mediated overexpression of transport proteins / Gary J. Litherland and Stephen A. Baldwin -- The amplified expression, identification, purification, assay, and properties of hexahistidine-tagged bacterial membrane transport proteins / Alison Ward [and others] -- Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters / Adrian R. Walmsley -- Detection and analysis of glucose transporters using photolabelling techniques / Alison K. Gillingham [and others] -- Computer prediction of transporter topology and structure / Rong-I Hong and Mark S.P. Sansom -- Two-dimensional crystallization of membrane proteins / Philippe Ringler, Bernard Heymann, and Andreas Engel -- Crystallization of membrane proteins / Tina D. Howard, Katherine E. McAuley-Hecht, and Richard J. Cogdell.
- 摘要: Membrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.
- 電子資源: https://dbs.tnua.edu.tw/login?url=https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98429
- 系統號: 005305467
- 資料類型: 電子書
- 讀者標籤: 需登入
- 引用網址: 複製連結
Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.
來源: Google Book
來源: Google Book
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