附註:Includes bibliographical references and index.
Protein expression in mammalian cells / Marlies Otter-Nilsson and Tommy Nilsson -- Expression in Xenopus oocytes and cell-free extracts / Glenn M. Matthews -- Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris / Mick F. Tuite, Jeff J. Clare, and Mike A. Romanos -- Baculovirus expression systems / Claire L. Merrington, Linda A. King, and Robert D. Possee -- Protein synthesis in eukaryotic cell-free systems / Mike J. Clemens and Ger J.M. Pruijn -- Prokaryotic in vivo expression systems / Edward R. Appelbaum and Allan R. Shatzman -- Cell-free coupled transcription-translation systems from Escherichia coli / Gisela Kramer, Wieslaw Kudlicki, and Boyd Hardesty -- Monitoring protein expression / John Colyer.
摘要:Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
