資料來源: Google Book
Transcription factors :a practical approach
- 其他作者: Latchman, David S.
- 出版: Oxford ;New York : Oxford University Press 1999.
- 版本: 2nd ed.
- 稽核項: 1 online resource (xxii, 303 pages) :illustrations.
- 叢書名: The practical approach series ;201
- 標題: Manuels de laboratoire. , Transcription génétique Régulation -- Manuels de laboratoire. , SCIENCE Life Sciences -- Molecular Biology. , Transcription Factors , Life SciencesMolecular Biology. , Genetic transcription , Transcription génétique , Facteurs de transcription , Régulation , Transcription factors , Transcription factors. , SCIENCE , Transcription factors Laboratory manuals. , Electronic books. , Facteurs de transcription Manuels de laboratoire. , physiology , Transkription , Genetic transcription Regulation -- Laboratory manuals. , Regulation , Methode , Laboratory manuals. , Transcription Factors physiology , Transkriptionsfaktor
- ISBN: 0191565792 , 9780191565793
- 試查全文@TNUA:
- 附註: Includes bibliographical references and index.
- 摘要: "Since publication of the first edition of this book five years ago, a wide range of new methodologies have been developed to facilitate studies on transcription factors. The book has been radically updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have either been fully updated or rewritten and additional chapters now included cover the use of in vitro transcription assays, analysis of the effect of transcription factors on chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications." "Each chapter includes full step-by-step protocols for immediate use at the laboratory bench and innumerable hints and tips for success. Anyone studying transcription factors in a research setting should find this book to be an invaluable bench companion." --Book Jacket.
- 電子資源: https://dbs.tnua.edu.tw/login?url=https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98483
- 系統號: 005305521
- 資料類型: 電子書
- 讀者標籤: 需登入
- 引用網址: 複製連結
Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by both experimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then the following chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting the phosphorylation and glycosylation state of transcription factors.
來源: Google Book
來源: Google Book
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