附註:Includes bibliographical references and index.
1. Assaying inositol phospholipid turnover in plant cells-- 2. Measurement of inositol phosphate turnover in intact cells and cell-free systems-- 3. Mass assay of inositol and its use to assay inositol polyphosphates-- 4. Expression, purification and interfacial kinetic analysis of phospholipase C-[gamma]1-- 5. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-[beta] isoenzymes-- 6. Purification and assay of PLC-Ơ-- 7. The Purification and assay of inositide binding proteins.
8. Mass assay of inositol 1,4,5-triphosphate and phospatidylinositol 4,5-bisphospate-- 9. Desalting inositol polyphospates by dialysis-- 10. The Ins(1,4,5)P₃ receptor-- 11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase-- 12. Phosphoinositide 3-kinase-- A1. List of suppliers-- A2. List of suppliers of inositol derivatives.
摘要:"Inositide research has infiltrated numerous branches of biochemistry, physiology, and molecular biology. Consequently, methodological information has been scattered far and wide. This book unites a wide selection of the most fundamental and commonly used techniques. Contributors come from international signal transduction laboratories that have been actively involved in developing and refining these techniques. A unique feature of the book is a catalog of non-commercial sources of synthetic inositide analogues. Because there's an emphasis on broader applications instead of the more esoteric methods, this latest volume in the Practical Approach series is useful as an introduction and a refresher for new and seasoned inositologists, respectively."--Publisher.
