附註:Includes bibliographical references and index.
The cytoskeleton in the transduction of signal and regulation of cellular function / Coralie A. Carothers Carraway -- Assaying binding and covalent modifications of cytoskeletal proteins / Christophe Ampe and Joël Vandekerckhove -- Methods to study actin assembly and dynamics in yeast / Tatiana S. Karpova and John A. Cooper -- Signalling complexes : association of signalling proteins with the cytoskeleton / Coralie A. Carothers Carraway and Kermit L. Carraway -- Ras-related GTPases and the cytoskeleton / Anne J. Ridley and Helen M. Flinn -- Applications for analysis of contractile functions in dividing and migrating tissue culture cells / Douglas J. Fishkind [and others] -- Video microscopy : protocols for examining the actin-based motility of Listeria, Shigella, vaccinia, and lanthanum-induced endosomes / W.L. Zeile, D.L. Purich, and F.S. Southwick -- Structural studies on tubulin and microtubules / Kenneth H. Downing -- Regulation of intermediate filament dynamics : a novel approach using site- and phosphorylation state-specific antibodies / Hiroyasu Inada [and others] -- The study of mRNA-cytoskeleton interactions and mRNA sorting in mammalian cells / Giovanna Bermano and John E. Hesketh -- Cell shape control and mechanical signalling through the cytoskeleton / Wolfgang H. Goldmann [and others].
摘要:This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these burgeoning fields.
