附註:Includes bibliographical references (pages 229-231) and index.
Chapter 1 Introduction: The use of animal cell culture -- chapter 2 Characteristics of cells in culture -- chapter 3 Basic equipment and laboratory design: what you need to get started in cell culture -- chapter 4 Growth and maintenance of cells in culture -- chapter 5 Cell line and culture monitoring -- chapter 6 Genetic engineering of animal cells in culture -- chapter 7 The glycosylation of proteins in cell culture -- chapter 8 Hybridomas--sources of antibodies -- chapter 9 Scaling up animal cell culture -- chapter 10 Modes of culture for high cell densi -- chapter 11 Production from cell culture -- chapter 12 Mammalian cell products: established and potential.
摘要:This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for scientists intending to initiate some work in this field. New topics are included to reflect the latest developments and trends in the field. They cover the latest theory of the biological clock (teleomeres) of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation and the humanization of antibodies for therapeutic use.
