附註:Includes bibliographical references and index.
Follicle development in vitro / Norah Spears, Evelyn E. Telfer and Alison A. Murray -- Preparation of gametes, in vitro maturation, in vitro fertilization, and embryo recovery and transfer / Michelle Lane and David K. Gardner -- Culture of the mammalian preimplantation embryo / David K. Gardner and Michelle Lane -- Assessment of preimplanation embryo development and viability / Denny Sakkas, Michelle Lane and David K. Gardner -- Micromanipulation of gametes / Gianpiero D. Palermo [and others] -- Micromanipulation: biopsy / Joyce C. Harper and Alpesh Doshi -- Analysis of intracellular ions in embryos: pH and calcium / Michelle Lane, Jay M. Baltz, and David K. Gardner -- Assessment of nutrient uptake, metabolite production, and enzyme activity / David K. Gardner and Michelle Lane -- Metabolic pathway activity / Don Rieger -- Confocal imaging of structural molecules in mammalian gametes / Calvin Simerly [and others].
Fluorescent in situ hybridization for dection of aneuploidy in single human blastomeres for preimplanation genetic diagnosis / Leeanda Wilton -- Assessment of ploidy, telomere length, and telomerase activity in oocytes and embryos / W. Allan King [and others] -- Microscale RNA isolation from mammalian embryos / C. Wrenzycki, Andrew J. Watson, and Michele Calder -- Relative mRNA transcript abundance in early embryos by reverse transcription-polymerase chain reaction / Paul A. de Sousa and Andrew J. Watson.
Characterization of novel genes during early development by application of differential display RT-PCR / David R. Nataleand Andrew J. Watson -- Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells / Keith E. Latham [and others].
Antisense disruption of gene expression in the preimplantation embryo / Lisa C. Barcroft, D. Holstead Jones-Taggart and Andrew J. Watson -- Gene subtraction and analysis / Marc-André and Claude Robert -- Analysis of apoptosis in the preimplantation embryo / Daniel R. Brison [and others] -- Cytoplasmic signaling and cell cycle control in the mouse egg and embryo / D. Page Baluch, Christine M. Pauken, and David G. Capco -- Human embryonic stem cells / Susan E. Lazendorf, Catherine A. Boyd, and Diane L. Wright -- Spermatogonial transplantion / Makoto Nagano -- Somatic cell nuclear transfer / Paul de Sousa [and others] -- Cryobiology: slow freezing vitrification of embryos / Tetsunori Mukaida and Magosaburo Kasai.
摘要:This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as "Manipulating the Mouse Embryo", from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.
